Mechanism for a Directional, Processive, and Reversible DNA Motor
نویسندگان
چکیده
منابع مشابه
The diffusive search mechanism of processive myosin class-V motor involves directional steps along actin subunits.
It is widely accepted that the vesicle-transporter myosin-V moves processively along F-actin with large steps of approximately 36 nm using a hand-over-hand mechanism. A key question is how does the rear head of two-headed myosin-V search for the forward actin target in the forward direction. Scanning probe nanometry was used to resolve this underlying search process, which was made possible by ...
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A procedure has been developed to assess whether polymerization of nucleotides by DNA polymerases is processive, that is, whether a succession of polymerization steps occurs without release of the enzyme from the template. The method involves measurement of the ratio of deoxyguanylate to deoxycytidylate incorporated in the course of replicating a segment of the right hand cohesive end of phage ...
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Comparison of the rates of polymerization catalyzed by DNA polymerases in the presence of a limited and a complete complement of the four deoxynucleoside triphosphates permits accurate measurement of the processivity of polymerization. The extent of processivity, Le. the number of nucleotides polymerized each time the DNA polymerase associates with the DNA template, may be measured in a range f...
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ژورنال
عنوان ژورنال: Small
سال: 2009
ISSN: 1613-6810,1613-6829
DOI: 10.1002/smll.200900078